A PDZ domain-based assay for measuring HIV protease activity: Assay design considerations
نویسندگان
چکیده
منابع مشابه
Assay design considerations A PDZ domain-based assay for measuring HIV protease activity: References
We have recently described a biochemical detection method for peptide products of enzymatic reactions based on the formation of PDZ domain·peptide ligand complexes. The product sensor is based on using masked or cryptic PDZ domain peptide ligands as enzyme substrates. Upon enzymatic processing, a PDZ-binding motif is exposed, and the product sequence bound specifically by a Euchelate-labeled GS...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2003
ISSN: 0961-8368,1469-896X
DOI: 10.1110/ps.0235603